Glycosaminoglycan-based biohybrid hydrogels are highly promising materials for tissue engineering and regenerative medicine due to their ability to provide cell-instructive environments. In this article, Jana Sievers-Liebschner, Ron Dockhorn, Jens Friedrichs, Thomas Kurth, Peter Fratzl, Jens-Uwe Sommer, Carsten Werner, and Uwe Freudenberg investigate the nanoscale molecular network structure of these hydrogels using an integrated analytical approach.
The study combines transmission electron microscopy, X-ray scattering, computer simulations, and AFM-based nanoindentation to quantitatively characterize nanoscale polymer network connectivity and structural inhomogeneities. These parameters are essential for understanding hydrogel mechanics, growth factor delivery, and cell–material interactions relevant to regenerative therapies and organoid culture systems.
Atomic force microscopy (AFM)-based nanoindentation measurements were performed to determine the mechanical stiffness of the hydrogels in both PBS and ethanol environments. Measurements were conducted using a modified NanoWorld PNP-TR-TL-Au AFM probe equipped with a 10 μm silica bead for colloidal probe nanoindentation.
Nanoindentation experiments were carried out using a set point of 6 nN and an approach/retract velocity of 5 μm/s. At least 70 force–distance curves were recorded for each sample at different positions across the hydrogel surface. Young’s modulus values were extracted using the Hertz model, enabling quantitative evaluation of hydrogel nanomechanical properties.
This work demonstrates how AFM-based nanoindentation with a NanoWorld AFM probe contributes to the detailed characterization of biohybrid hydrogel networks and supports the development of engineered matrices for biomedical applications.
Fig. 5. Computational modelling of starPEG-heparin hydrogel networks. A: Simulation snapshots of hydrated and dehydrated starPEG-heparin hydrogels. For the hydrated network (A1), a good solvent (equivalent to PBS) was assumed. To model the dehydrated state (A2), parameters were adjusted to promote the self-aggregation of starPEG molecules in a poor solvent (e.g., ethanol). Networks with different effective molar ratios after crosslinking (γBMC, where BMC is the Biggest Molecule Cluster) are shown, assuming a 90 % extent of reaction between starPEG (grey) and heparin (yellow). Cube size: L = 150 nm. B: Molar ratio of the BMC γBMC after crosslinking at an extent of reaction p = 0.9, plotted as a function of the initial molar ratio. The dotted line represents the theoretical ideal value, while the blue line shows the experimentally determined Young’s moduli as a function of crosslinking degree. C: Incorporation efficiency of starPEG and heparin within the BMC, calculated as the number of starPEG or heparin molecules in the BMC divided by the number in the reaction mixture (ideal network). Insert: Total number of starPEG or heparin molecules in the reaction mixture (initial) or within the BMC. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Full citation:
Sievers-Liebschner, J.; Dockhorn, R.; Friedrichs, J.; Kurth, T.; Fratzl, P.; Sommer, J.-U.; Werner, C.; Freudenberg, U. Unravelling the molecular network structure of biohybrid hydrogels.
Materials Today Bio 2025, 34, 102249. https://doi.org/10.1016/j.mtbio.2025.102249
Alzheimer’s disease (AD) is the most frequent neurodegenerative disorder in the elderly aged over 65.*
The extracellular accumulation of beta-amyloid (Aβ) aggregates in the brain is considered as the major event worsening the AD symptoms, but its underlying reason has remained unclear.
In the article „Lateral Piezoelectricity of Alzheimer‘s Aβ Aggregates“ by Jinhyeong Jang, Soyun Joo, Jiwon Yeom, Yonghan Jo, Jingshu Zhang, Seungbum Hong and Chan Beum Park the piezoelectric characteristics of Aβ aggregates are revealed.
The vector piezoresponse force microscopy (PFM) analysis results exhibit that Aβ fibrils have spiraling piezoelectric domains along the length and a lateral piezoelectric constant of 44.1 pC N-1. Also, the continuous sideband Kelvin probe force microscopy (KPFM) images display that the increment of charge-induced surface potential on a single Aβ fibril is allowed to reach above +1700 mV in response to applied forces.
These findings shed light on the peculiar mechano-electrical surface properties of pathological Aβ fibrils that exceed those of normal body components.*
Both KPFM and nanoindentation measurements were performed using a chemically inert conductive diamond AFM tip. (NanoWorld PointprobeCDT-FMR).
Vertical and lateral PFM images of Aβ fibrils were obtained by scanning probe microscopy with a platinum/iridium-coated silicon cantilever ( NanoWorld Pointprobe®CONTPt ). The scanning angle of the PFM cantilever was set to zero, with scan rate of 0.5 Hz and loading force of 10 nN. This setup minimized electrostatic contributions to the torsional movement of the tip, allowing measurements to focus on the shear electromechanical effects of Aβ fibrils. Vertical and lateral drive frequencies were 53 kHz and 309 kHz, respectively.*
Figure S10 from „Lateral Piezoelectricity of Alzmeiner‘s Aβ Aggregates by Jinhyeong Jang et al.: Nanoindentation test result of Aβ fibrils. Direct piezoelectric effects are tested by measuring the surface potential change before and after nano-indentation of Aβ fibrils. (A) Schematic illustration of the nanoindentation test for Aβ fibrils coated on an Au/Cr substrate. (B) Topgraphy of Aβ fibrils acquired before and after applying nanoindentation. The post-indentation image shows white spots resulting from physical interactions between the AFM tip and the sample, while the approximate structure of the Aβ fibrils remains intact. Height and potential line sections obtained fom the (c) blue and (d) red lines are shown in the topgraphy of Aβ fibrils. The height remains unchanged, while electrical voltage increment and decrement were observed at the blue and red lines, respectively. (D) During the nanoindentation the Young‘s modulus of Aβ – fibrils was measured to be 3.17 GPa, closely approxmating the literature (Nanoscale 4, 4426-4429 (2012). Both KPFM and nanoindentation measurements were performed using a chemically inert conductive diamond AFM tip (CDT-FMR, NanoWorld, Switzerland). Collected force curves were analyzed using the Hertz model to estimate the elastic moduli.
*Jinhyeong Jang, Soyun Joo, Jiwon Yeom, Yonghan Jo, Jingshu Zhang, Seungbum Hong and Chan Beum Park Lateral Piezoelectricity of Alzheimer‘s Aβ Aggregates
Advanced Science, Volume 11, Issue 39, October 024, 2406678
DOI: https://doi.org/10.1002/advs.202406678
Open Access The article “Lateral Piezoelectricity of Alzheimer‘s Aβ Aggregates” byJinhyeong Jang, Soyun Joo, Jiwon Yeom, Yonghan Jo, Jingshu Zhang, Seungbum Hong and Chan Beum Park is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Airflow limitation in obstructive airway disease is characterized by narrowing of the airway lumen from excessive contraction of airway smooth muscle (ASM) and remodeling of the airway wall which includes changes in the extracellular matrix (ECM) of the ASM layer.*
Previous studies on human airway smooth muscle cells ( hASMC ) have independently assessed the influence of extracellular matrix (ECM) proteins on substrates of supra-physiological stiffnesses, such as tissue culture plastic or glass.*
While the influence of discrete substrate stiffness on hASMC behavior has been examined, manipulation of both substrate stiffness and ECM proteins simultaneously (as expected in disease) has not been extensively modeled in vitro.*
In the article “Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels” Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi highlight the interplay and complexities between stiffness and ECM protein type on hASMC mechanosensation, relevant to airway remodelling in obstructive airway diseases.*
The authors first determined a physiological range of ASM layer stiffness using a porcine airway and used these empirical recordings to inform the fabrication of a linear stiffness gradient platform coated with different ECM proteins.*
Using this linear stiffness gradient platform, Yong Hwee Tan et al. profiled hASMC morphology, contractile function with alpha-smooth muscle actin (αSMA) and mechanisms of mechanosensation, specifically with nuclear translocalization of Yes-associated protein (YAP) and lamin-A expression.*
Yong Hwee Tan et al.’s assessment of hASMC mechanosensation utilized an innovative hydrogel platform delivering a linear stiffness gradient to understand stiffness-mediated cell behavior with an ECM substrate for cellular adhesion. *
The employment of a stiffness gradient that was designed after empirical measurements performed on ex vivo ASM tissue, enabled the presentation of physiologically relevant stiffnesses to study hASMC behavior.*
Using this platform, the authors of the article found that hASMC mechanosense underlying mechanical cues more than the types of proteins they are anchored to by screening hASMC morphology, contractile phenotype, and mechanomarker expression, with a few exceptions.*
While the authors acknowledge that the findings from their study were done using cells from only one donor they still think that their study provides a proof of concept for the relevance of hASMC mechanosensation to ECM stiffness, and is another step in the right direction for understanding the pathophysiological impact of airway remodeling in obstructive diseases and exploring potential avenues for improving therapy through greater fidelity of in vitro platforms that include key concepts of mechanosensation. *
Yong Hwee Tan et al. wanted to use the same method which is used to assess hydrogel stiffness, namely atomic force microscopy (AFM), to measure ASM stiffness.*
However, nanoscale measurements of ASM strips by AFM proved to be difficult due to an uneven tissue surface after de-epithelialization (Figure S1C, Supporting Information of the cited article), resulting in false force triggering.
To validate the translation of stiffness values measured from macroscale compression (ASM strips) to nanoscale indentation (AFM on hydrogels), Yong Hwee Tan et al. fabricated additional hydrogels of four different stiffnesses using well-characterized polyacrylamide and compared the stiffness of hydrogels measured by uniaxial compression tester and atomic force microscopy (Figure S2A, Supporting Information of the cited article).
The nanoscale stiffness of hydrogels was assessed using an atomic force microscope (AFM) with NanoWorld triangular Pyrex-NitridePNP-TR AFM probes (the longer AFM cantilever beam – CB 2 – with 200 µm length was used).
These AFM tips probed hydrogels immersed in 1 × PBS with 2 nN, an approach velocity of 2 µm s−1 and a retraction velocity of 10 µm s−1.
Young’s modulus was determined from linear portions of contact-generated force curves using a custom-written code in Igor Pro.
All probe indentations were made in triplicate and averaged for a stiffness measurement in kilopascals (kPa).
An example force curve is shown in Figure S2B, Supporting Information of the cited article. Validation of a linear stiffness gradient was achieved with eight indentations on the hydrogel, 2 mm away from both edges of the hydrogel and at 1 mm intervals along the stiffness gradient axis. Measurements were plotted against displacement from the hydrogel edge (soft to stiff) (Figure 2B of the cited article).
Figure 2 from Yong Hwee Tan et al. 2024 “Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels”: Linear stiffness gradient hydrogel fabrication. A) A schematic of a two-step polymerization process. i) 120 µL of mixed polyacrylamide (PA) solution (% acrylamide + % bis-acrylamide) was added to the primary mold and left to polymerize under ii) a methacrylated coverslip for 20 min. iii) Wedge-shaped 1° gel was removed and flipped for placement of a iv) secondary mold before v) addition of a second 120 µL PA solution and polymerized under a vi) dichlorodimethylsilane-coated coverslip for 20 min. vii) Removal of coverslip and mold completes the fabrication of bi-layered stiffness gradient hydrogel. viii) the dotted arrow indicating the direction of gradient and atomic force microscopy (AFM) measurement. B) Young’s moduli gradient measured by AFM. Twelve hydrogels were selected (one gel per batch) and assessed for stiffness, yielding a gradient of 4.0 kPa mm−1, with a range of 1.7 ± 1.2 to 29.6 ± 4.3 kPa (R2 = 0.998, n = 8). Data are presented as mean ± SD.
Figure S2 from Yong Hwee Tan et al. 2024 “Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels”: (A) Correlation of Young’s modulus from macroscale stiffness (UCT) assessment with nanoindentation (AFM), was conducted using cylindrical PA hydrogels of different Acrylamide %/Bis-acrylamide % derived from Tse and Engler [47] 10 %/0.06 %, 10 %/0.1 %, 10 %/0.15 % and 10 %/0.3 % (Linear regression, P < 0.0001, R2 = 0.9288, n = 4). Data are presented as mean SEM. (B) An example force curve from atomic force microscopy with an approach velocity of 2 μm/s, until a 2 nN trigger force was registered, and retraction of indenter at 10 μm/s.*Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels
Advanced Healthcare Materials 2024, 2304254
DOI: https://doi.org/10.1002/adhm.202304254
The article “Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels” by Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
![Figure S2 from Yong Hwee Tan et al. 2024 “Stiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels”:(A) Correlation of Young’s modulus from macroscale stiffness (UCT) assessment with nanoindentation (AFM), was conducted using cylindrical PA hydrogels of different Acrylamide %/Bis-acrylamide % derived from Tse and Engler [47] 10 %/0.06 %, 10 %/0.1 %, 10 %/0.15 % and 10 %/0.3 % (Linear regression, P < 0.0001, R2 = 0.9288, n = 4). Data are presented as mean SEM. (B) An example force curve from atomic force microscopy with an approach velocity of 2 μm/s, until a 2 nN trigger force was registered, and retraction of indenter at 10 μm/s. NanoWorld triangular Pyrex-Nitride PNP-TR AFM probes were used to assess the stiffness of the hydrogels with atomic force microscopy.](https://www.nanoworld.com/blog/wp-content/uploads/2024/07/Fig-S2-from-Young-Hwee-Tan-2024-Stiffness-Mediated-Mechanosensation-of-Airway-Smooth-Muscle-Cells-on-Linear-Stiffness-Gradient-Hydrogels-NanoWorld-PNP-TR-AFM-probe.jpg)