Arrow TL2

MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics

High metastasis-associated in colon cancer 1 (MACC1) expression is associated with metastasis, tumor cell migration, and increased proliferation in colorectal cancer. Tumors with high MACC1 expression show a worse prognosis and higher invasion into neighboring structures. However, the mediation of the pro-migratory effects is still a matter of investigation.*

In their study “MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics” Tim Hohmann, Urszula Hohmann, Mathias Dahlmann, Dennis Kobelt, Ulrike Stein and Faramarz Dehghani aim to elucidate the impact of single cell biomechanics and proliferation on MACC1-dependent migration.*

The authors found that MACC1 expression associated with increased collective migration, caused by increased proliferation, and no changes in single cell biomechanics. Thus, targeting proliferation in high-MACC1-expressing tumors may offer additional effects on cell migration.*

The mechanical properties of single cells were assessed in the form of the Young’s modulus and cortex tension; both were measured using atomic force microscopy. Briefly, cells were seeded on a petri dish and measured 15 min after seeding to avoid slippage of individual cells. Measurements were conducted using a tipless NanoWorld Arrow-TL2 AFM cantilever array to apply a force of 1 nN that led to deformations of 1–2 µm. The Young’s modulus was calculated using the Hertz model.*

Figure 1 from “MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics” by Tim Hohmann et al. Single cell properties of high- and low-MACC1-expressing colon carcinoma cells. (A,B) depict the results of the biomechanical measurements for the Young´s modulus and the cortex tension. (C,D) show the results of live cell imaging of single cells for the mean speed and the contact area with the substrate. Sample sizes: (A) nSW480/EV = 35; nSW480/MACC1 = 33; nSW620/shMACC1 = 40; nSW620/shCTL = 40. (B) nSW480/EV = 33; nSW480/MACC1 = 31; nSW620/shMACC1 = 25; nSW620/shCTL = 26. (C,D) nSW480/EV = 66; nSW480/MACC1 = 98; nSW620/shMACC1 = 102; nSW620/shCTL = 111. Asterisk depicts statistically significant results with p < 0.05. Box plots show the median (red line), 25 and 75 percentile (box), non-outlier range (whiskers), and outliers (red dots). The mechanical properties of single cells were assessed in the form of the Young’s modulus and cortex tension; both were measured using atomic force microscopy. Briefly, cells were seeded on a petri dish and measured 15 min after seeding to avoid slippage of individual cells. Measurements were conducted using a NanoWorld Arrow-TL2 AFM cantilever to apply a force of 1 nN that led to deformations of 1–2 µm. The Young’s modulus was calculated using the Hertz model. — Figure 1 from “MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics” by Tim Hohmann et al.
Single cell properties of high- and low-MACC1-expressing colon carcinoma cells. (A,B) depict the results of the biomechanical measurements for the Young´s modulus and the cortex tension. (C,D) show the results of live cell imaging of single cells for the mean speed and the contact area with the substrate. Sample sizes: (A) nSW480/EV = 35; nSW480/MACC1 = 33; nSW620/shMACC1 = 40; nSW620/shCTL = 40. (B) nSW480/EV = 33; nSW480/MACC1 = 31; nSW620/shMACC1 = 25; nSW620/shCTL = 26. (C,D) nSW480/EV = 66; nSW480/MACC1 = 98; nSW620/shMACC1 = 102; nSW620/shCTL = 111. Asterisk depicts statistically significant results with p < 0.05. Box plots show the median (red line), 25 and 75 percentile (box), non-outlier range (whiskers), and outliers (red dots).

*Tim Hohmann, Urszula Hohmann, Mathias Dahlmann, Dennis Kobelt, Ulrike Stein and Faramarz Dehghani
MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics
Cancers 2022, 14(12), 2857
DOI: https://doi.org/10.3390/cancers14122857

Open Access

The article “MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics” by Tim Hohmann, Urszula Hohmann, Mathias Dahlmann, Dennis Kobelt, Ulrike Stein and Faramarz Dehghani is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

Optimized positioning through maximized tip visibility – Arrow AFM probes screencast passes 500 views mark

The screencast about NanoWorld Arrow Silicon AFM probes held byNanoWorld AG CEO Manfred Detterbeck has just passed the 500 views mark. Congratulations Manfred!

NanoWorld Arrow™ AFM probes are designed for easy AFM tip positioning and high resolution AFM imaging and are very popular with AFM users due to the highly symetric scans that are possible with these AFM probes because of their special tip shape. They fit to all well-known commercial SPMs (Scanning Probe Microscopes) and AFMs (Atomic Force Microscopes). The Arrow AFM probe consists of an AFM probe support chip with an AFM cantilever which has a tetrahedral AFM tip at its triangular free end.

The Arrow AFM probe is entirely made of monolithic, highly doped silicon.

The unique Arrow™ shape of the AFM cantilever with the AFM tip always placed at the very end of the AFM cantilever allows easy positioning of the AFM tip on the area of interest.
The Arrow AFM probes are available for non-contact mode, contact mode and force modulation mode imaging and are also available with a conductive platinum iridum coating. Furthermore the Arrow™ AFM probe series also includes a range of tipless AFM cantilevers and AFM cantilever arrays as well as dedicated ultra-high frequency Arrow AFM probes for high speed AFM.

To find out more about the different variations please have a look at:

https://www.nanoworld.com/arrow-afm-tips

You can also find various application examples for the Arrow AFM probes in the NanoWorld blog. For a selection of these articles just click on the “Arrow AFM probes” tag on the bottom of this blog entry.

Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP

Quantifying the adaptive mechanical behavior of living cells is essential for the understanding of their inner working and function.*

In their article “Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP” Mark Skamrahl, Huw Colin‐York, Liliana Barbieri and Marco Fritzsche use a combination of atomic force microscopy and fluorescence recovery after photobleaching is introduced which offers simultaneous quantification and direct correlation of molecule kinetics and mechanics in living cells.*

Simultaneous quantification of the relationship between molecule kinetics and cell mechanics may thus open up unprecedented insights into adaptive mechanobiological mechanisms of cells.*

For the AFM nanoindentation tests described in their publication the authors used NanoWorld Arrow-TL2 tipless cantilevers that were functionalized with a polystyrene bead with 5 µm radius.*

Figure 1 a from “Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP” by M. Skamrahl et al.:
Establishment and calibration of the optomechanical AFM–FRAP platform. a) Schematic of the AFM–FRAP setup illustrating the experimental power of simultaneous quantification of molecule kinetics and cell mechanics

*Mark Skamrahl, Huw Colin‐York, Liliana Barbieri, Marco Fritzsche
Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP
Small 2019, 1902202
DOI: https://doi.org/10.1002/smll.201902202

Please follow this external link to the full article https://onlinelibrary.wiley.com/doi/full/10.1002/smll.201902202

Open Access: The article « Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP » by Mark Skamrahl, Huw Colin‐York, Liliana Barbieri and Marco Fritzsche is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other thirdparty material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.