Type: PNP-DB

Pyrex-Nitride Probe – Diving Board AFM Cantilevers

Logo
Cantilever Data* Cant. 1 Cant. 2
Shape Beam
Resonance Frequency 67 kHz 17 kHz
Force Constant 0.48 N/m 0.06 N/m
Length 100 µm 200 µm
Mean Width 40 µm 40 µm
Thickness 500 nm 500 nm
Pyrex-Nitride oxide sharpened, pyramidal tip

Pyrex-Nitride oxide sharpened, pyramidal tip More images

Product Description

Leading edge in sharpness and durability

The Pyrex-Nitride AFM probes have silicon nitride AFM cantilevers with very low force constants and integrated oxide sharpened, pyramidal AFM tips with a height of 3.5 µm. The AFM tip is located 4µm behind the free end of the AFM cantilever. This AFM probe series features a support chip that is made of Pyrex. Two chip versions are available: The DB series with rectangular / diving board AFM cantilevers and the TR series having triangular AFM cantilevers.

All chips are pre-separated prior to shipment and come in Gel-Pak containers.

The typical AFM tip radius of curvature of is below 10 nm.

Tip shape: Pyramid

Coating: Reflective Gold

Gold Reflex Coating

The gold reflex coating consists of a 70 nm thick gold layer deposited on the detector side of the AFM cantilevers which enhances the reflectance of the laser beam. Furthermore it prevents light from interfering within the AFM cantilever.

As the coating is almost stress-free the bending of the AFM cantilevers due to stress is less than 2 degrees.

Order Codes

Order Code Quantity Data Sheet
PNP-DB-20 20 yes
PNP-DB-50 50 yes

NanoWorld® Pyrex-Nitride AFM Probes (PNP) Screencast

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Scientific publications mentioning use of this AFM probe

Byvalov, A. A., V. S. Belozerov, I. V. Konyshev, and B. A. Ananchenko.
Comparative Assessment of the Force Characteristics of the Interaction of the Yersinia pseudotuberculosis Lipopolysaccharide with Antibodies by Optical Trapping and Atomic Force Microscopy
Biophysics. 2024 Oct;69(5):828-35
DOI: https://doi.org/10.1134/S000635092470091X


Lee, Hoyeon, Boyoung Kim, Jiyue Yun, Jinseung Bae, Sungsu Park, Junseok Jeon, Hye Ryoun Jang, Jaecheol Lee, and Soah Lee
PIV-MyoMonitor: an accessible particle image velocimetry-based software tool for advanced contractility assessment of cardiac organoids
Frontiers in Bioengineering and Biotechnology. 2024 Mar 12;12:1367141
DOI: https://doi.org/10.3389/fbioe.2024.1367141


Cava, Daniel G., Julia Alvarez-Malmagro, Paolo Natale, Sandra López-Calcerrada, Iván López-Montero, Cristina Ugalde, Jose Maria Abad, Marcos Pita, Antonio L. De Lacey, and Marisela Vélez
Electrochemical detection of quinone reduced by Complex I Complex II and Complex III in full mitochondrial membranes
Electrochimica Acta. 2024 Apr 20;484:144042
DOI: https://doi.org/10.1016/j.electacta.2024.144042


McNeilly S, Thomson CR, Gonzalez-Trueba L, Sin YY, Granata A, Hamilton G, Lee M, Boland E, McClure JD, Lumbreras-Perales C, Aman A
Collagen IV deficiency causes hypertrophic remodeling and endothelium-dependent hyperpolarization in small vessel disease with intracerebral hemorrhage
EBioMedicine. 2024 Sep 1;107
DOI: https://doi.org/10.1016/j.ebiom.2024.105315


García‐Arribas AB, Ibáñez‐Freire P, Carlero D, Palacios‐Alonso P, Cantero‐Reviejo M, Ares P, López‐Polín G, Yan H, Wang Y, Sarkar S, Chhowalla M.
Broad Adaptability of Coronavirus Adhesion Revealed from the Complementary Surface Affinity of Membrane and Spikes
Advanced Science. 2024 Nov;11(41):2404186.
DOI: https://doi.org/10.1002/advs.202404186


Belozerov, V. S., B. A. Ananchenko, I. V. Konyshev, L. G. Dudina, S. A. Konnova, E. V. Rozhina, R. F. Fakhrullin, and A. A. Byvalov
Force Characteristics of Yersinia pestis Lipopolysaccharide Interaction with TLR4 and CD14 Receptors on J774 Macrophages: Atomic Force Microscopy
 Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 2023 Sep;17(3):200-7
DOI: https://doi.org/10.1134/S1990747823040037


Álvarez-López, Aroa, Raquel Tabraue-Rubio, Sandra Hernández-Escobar, Rafael Daza, Luis Colchero, Parsa Rezvanian, Manuel Elices, Gustavo V. Guinea, Daniel González-Nieto, and José Pérez-Rigueiro
Application of single cell force spectroscopy (SCFS) to the assessment of cell adhesion to peptide-decorated surfaces
International Journal of Biological Macromolecules. 2023 Jul 31;244:125369.
DOI: https://doi.org/10.1016/j.ijbiomac.2023.125369


Su, Yiqun, Tiantian Wang, Fan Zhang, Junsen Huang, Zhehang Zhu, Faiz Ullah Shah, Feng Xu, and Rong An
Effect of electrode surface chemistry on ion structuring of imidazolium ionic liquids
Langmuir. 2023 Jun 8;39(24):8463-74.
DOI: https://doi.org/10.1021/acs.langmuir.3c00710


Peng, Baoxu, Bingbing Li, Aimin Ge, Chengyang Xu, Ken-ichi Inoue, and Shen Ye
Role of Oxygen in the Formation of the Solid-Electrolyte Interphase Evaluated by Online Electrochemical Mass Spectrometry and Electrochemical Atomic Force Microscopy
The Journal of Physical Chemistry C. 2023 Jun 21;127(26):12528-40
DOI: https://doi.org/10.1021/acs.jpcc.3c02726


Budai, Nico, Hironari Isshiki, Ryota Uesugi, Zheng Zhu, Tomoya Higo, Satoru Nakatsuji, and YoshiChika Otani
High-resolution magnetic imaging by mapping the locally induced anomalous Nernst effect using atomic force microscopy
Applied Physics Letters. 2023 Mar 6;122(10)
DOI: https://doi.org/10.1063/5.0136613


Byvalov, A. A., V. S. Belozerov, B. A. Ananchenko, and I. V. Konyshev
Specific and nonspecific interactions of Yersinia pseudotuberculosis lipopolysaccharide with monoclonal antibodies assessed by atomic force microscopy
Biophysics. 2022 Dec;67(6):856-66
DOI: https://doi.org/10.1134/S0006350922060033


Cimmino, Chiara, Paolo A. Netti, and Maurizio Ventre
A switchable light-responsive azopolymer conjugating protein micropatterns with topography for mechanobiological studies
Frontiers in Bioengineering and Biotechnology. 2022 Jul 22;10:933410
DOI: https://doi.org/10.3389/fbioe.2022.933410


Nalbach, Mathis, Franziska Chalupa-Gantner, Felix Spoerl, Victor de Bar, Benedikt Baumgartner, Orestis G. Andriotis, Shingo Ito, Aleksandr Ovsianikov, Georg Schitter, and Philipp J. Thurner
Instrument for tensile testing of individual collagen fibrils with facile sample coupling and uncoupling
Review of Scientific Instruments. 2022 May 1;93(5).
DOI: https://doi.org/10.1063/5.0072123


Mpoyi EN, Cantini M, Sin YY, Fleming L, Zhou DW, Costell M, Lu Y, Kadler K, García AJ, Van Agtmael T, Salmeron-Sanchez M.
Material-driven fibronectin assembly rescues matrix defects due to mutations in collagen IV in fibroblasts
Biomaterials. 2020 Sep 1;252:120090
DOI: https://doi.org/10.1016/j.biomaterials.2020.120090


Su, Xuelian, Haijing Zhou, Guangjie Bao, Jizeng Wang, Lin Liu, Qian Zheng, Manli Guo, and Jinting Zhang
Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy
Biology open. 2020 Mar 15;9(3):bio048108.
DOI: https://doi.org/10.1242/bio.048108


Nilsen, Madeleine, Fabian Port, Michael Roos, Kay-Eberhard Gottschalk, and Steffen Strehle
Facile modification of freestanding silicon nitride microcantilever beams by dry film photoresist lithography
Journal of Micromechanics and Microengineering. 2019 Jan 14;29(2):025014
DOI: https://doi.org/10.1088/1361-6439/aaf7e3


Hsu, Chi‐Nung, Pei‐Yuan Lee, Ho‐Yi Tuan‐Mu, Chen‐Yu Li, and Jin‐Jia Hu
Fabrication of a mechanically anisotropic poly (glycerol sebacate) membrane for tissue engineering
Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2018 Feb;106(2):760-70
DOI: https://doi.org/10.1002/jbm.b.33876


Bahwini, Tariq Mohana, Yongmin Zhong, Chengfan Gu, Zeyad Nasa, and Denny Oetomo
Investigating the mechanical properties of biological brain cells with atomic force microscopy
Journal of Medical Devices. 2018 Dec 1;12(4):041007.
DOI: https://doi.org/10.1115/1.4040995


Kwek, Jin W., Kaliramesh Siliveru, Shuying Cheng, Qisong Xu, and RP Kingsly Ambrose
Zein film functionalized atomic force microscopy and Raman spectroscopic evaluations on surface differences between hard and soft wheat flour
Journal of Cereal Science. 2018 Jan 1;79:66-72.
DOI: https://doi.org/10.1016/j.jcs.2017.09.012


Andriotis, Orestis G., Sylvia Desissaire, and Philipp J. Thurner.
Collagen fibrils: nature’s highly tunable nonlinear springs
ACS nano. 2018 Mar 12;12(4):3671-80.
DOI: https://doi.org/10.1021/acsnano.8b00837


Guex, A. G., C. D. Spicer, A. Armgarth, A. Gelmi, E. J. Humphrey, C. M. Terracciano, S. E. Harding, and M. M. Stevens
Electrospun aniline-tetramer-co-polycaprolactone fibers for conductive, biodegradable scaffolds
MRS communications. 2017 Sep;7(3):375-82.
DOI: https://doi.org/10.1557/mrc.2017.45


Murphy, Ryan W., Brian E. Farkas, and Owen G. Jones
Effect of crosslinking on the physical and chemical properties of β-lactoglobulin (Blg) microgels
Journal of colloid and interface science. 2017 Nov 1;505:736-44
DOI: https://doi.org/10.1016/j.jcis.2017.06.061


Pelillo, Chiara, Hilaria Mollica, Johannes A. Eble, Julius Grosche, Lea Herzog, Barbara Codan, Gianni Sava, and Alberta Bergamo
Inhibition of adhesion, migration and of α5β1 integrin in the HCT-116 colorectal cancer cells treated with the ruthenium drug NAMI-A
Journal of inorganic biochemistry. 2016 Jul 1;160:225-35.
DOI: https://doi.org/10.1016/j.jinorgbio.2016.02.025


Bernoulli, D., K. Häfliger, K. Thorwarth, G. Thorwarth, R. Hauert, and R. Spolenak
"Cohesive and adhesive failure of hard and brittle films on ductile metallic substrates: A film thickness size effect analysis of the model system hydrogenated diamond-like carbon (aC: H) on Ti substrates
Acta materialia. 2015 Jan 15;83:29-36.
DOI: https://doi.org/10.1016/j.actamat.2014.09.044


Sbaizero OR, DelFavero GI, Martinelli V, Long CS, Mestroni L
Analysis of long-and short-range contribution to adhesion work in cardiac fibroblasts: An atomic force microscopy study
Materials Science and Engineering: C. 2015 Apr 1;49:217-24
DOI: https://doi.org/10.1016/j.msec.2014.12.083

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