{"id":961,"date":"2018-10-05T15:47:38","date_gmt":"2018-10-05T14:47:38","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=961"},"modified":"2023-04-18T12:59:41","modified_gmt":"2023-04-18T11:59:41","slug":"membrane-sculpting-by-curved-dna-origami-scaffolds","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/membrane-sculpting-by-curved-dna-origami-scaffolds\/","title":{"rendered":"Membrane sculpting by curved DNA origami scaffolds"},"content":{"rendered":"<p>In the article &#8220;Membrane sculpting by curved DNA origami scaffolds&#8221; the authors show that &#8220;dependent on curvature, membrane affinity and surface density, DNA origami coats can indeed reproduce the activity of membrane-sculpting proteins such as BAR, suggesting exciting perspectives for using them in bottom-up approaches towards minimal biomimetic cellular machineries.&#8221;*<\/p>\n<p>The AFM images for this article were taken in high-speed AC mode using NanoWorld Ultra-Short Cantilevers of the <a href=\"https:\/\/www.nanoworld.com\/Ultra-Short-Cantilevers-USC-F0.3-k0.3.html\">USC-F0.3-k0.3<\/a> type.<\/p>\n<figure id=\"attachment_964\" aria-describedby=\"caption-attachment-964\" style=\"width: 1083px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-964\" src=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds.jpg\" alt=\"Supplementary Figure 5 b from Membrane sculpting by curved DNA origami scaffolds: Characterization of folded DNA origami nanoscaffolds. ( a ) Assembly of the folded bare origami structures L, Q, H was initially assessed via agarose gel (2%) electrophoresis analysis. Lanes containing marker DNA ladder (1kb) and M13 single - stranded p7249 sc affold (Sc) were also included. ( b ) Structure of folded bare origami L, Q and H was further validated using negative - stain transmission electron microscopy (TEM ; scale bar s : 100 nm ) and atomic force microscopy (AFM ; scale bar s : 200nm ).\" data-wp-pid=\"964\" width=\"1083\" height=\"775\" srcset=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds.jpg 1083w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-300x215.jpg 300w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-768x550.jpg 768w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-1024x733.jpg 1024w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-1200x859.jpg 1200w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-800x572.jpg 800w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2018\/10\/05150809\/Supplementary-figure-5-b-from-Membrane-sculpting-by-curved-DNA-origami-scaffolds-880x630.jpg 880w\" sizes=\"auto, (max-width: 1083px) 100vw, 1083px\" \/><\/a><figcaption id=\"caption-attachment-964\" class=\"wp-caption-text\">Supplementary Figure 5 b from &#8220;Membrane sculpting by curved DNA origami scaffolds&#8221;:<br \/>Characterization of folded DNA origami nanoscaffolds.<br \/>b) Structure of folded bare origami L, Q and H was further validated using negative-stain transmission electron microscopy (TEM; scale bars: 100 nm) and atomic force microscopy (AFM; scale bars: 200nm).<\/figcaption><\/figure>\n<p>*Henri G. Franquelim, Alena Khmelinskaia, Jean-Philippe Sobczak, Hendrik Dietz, Petra Schwille<br \/>\n<strong>Membrane sculpting by curved DNA origami scaffolds<\/strong><br \/>\nNature Communicationsvolume 9, Article number: 811 (2018)<br \/>\nDOI: https:\/\/doi.org\/10.1038\/s41467-018-03198-9<\/p>\n<p>Please follow this link to the full article: <a href=\"https:\/\/rdcu.be\/8zZi\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/rdcu.be\/8zZi<\/a><\/p>\n<p>Open Access: The article \u201cMembrane sculpting by curved DNA origami scaffolds\u201d by Franquelim et. al is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In the article &#8220;Membrane sculpting by curved DNA origami scaffolds&#8221; the authors show that &#8220;dependent on curvature, membrane affinity and surface density, DNA origami coats can indeed reproduce the activity of membrane-sculpting proteins such as BAR, suggesting exciting perspectives for using them in bottom-up approaches towards minimal biomimetic cellular machineries.&#8221;* The AFM images for this &hellip; <a href=\"https:\/\/www.nanoworld.com\/blog\/membrane-sculpting-by-curved-dna-origami-scaffolds\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\" >Membrane sculpting by curved DNA origami scaffolds<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[8,17,48,96,105,225,50,25,24,47,86,16,26,53,106,51],"class_list":["post-961","post","type-post","status-publish","format-standard","hentry","category-news","tag-afm-probe","tag-atomic-force-microscopy","tag-biology-afm-probes","tag-biophysics","tag-dna-nanostructures","tag-dna-origami","tag-high-speed-afm","tag-high-speed-scanning","tag-hs-afm","tag-life-science-afm-probes","tag-nanoscale-biophysics","tag-scanning-probe-microscopy","tag-ultrafast-scanning","tag-usc","tag-usc-f0-3-k0-3","tag-video-rate-afm"],"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/961","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=961"}],"version-history":[{"count":10,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/961\/revisions"}],"predecessor-version":[{"id":1459,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/961\/revisions\/1459"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=961"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=961"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}