![\"Fig.](\"https:\/\/www.nanoworld.com\/blog\/wp-content\/uploads\/2025\/02\/Fig-2-from-Souvik-Naskar-et-al-2024-Mechanism-for-Vipp1-spiral-formation-ring-biogenesis-and-membrane-repair-scaled.jpeg\")
<\/a>Vipp1 assembles dynamic networks of spirals, rings and sheets on membrane
a, F-AFM phase timecourse showing Vipp1 recruitment to the highly curved edge of membrane patches. Scan rate, 70\u2009Hz; 256 \u00d7 256 pixels. The area in the dashed box is enlarged in b. b, Spiral and ring formation localized to the membrane edge. Scan rate, 70\u2009Hz; 256 \u00d7 256 pixels. c, Left, phase timecourse showcasing a dense network of sheets, spirals, and rings that ultimately cover the entire membrane plane. Right, average of six F-AFM height images. Scan rate, 120\u2009Hz; 256 \u00d7 256 pixels. d, Average F-AFM height image showing Vipp1 sheet, spiral, and ring detail. Red arrows mark the sheet branching into filaments ~13\u2009nm wide. Scan rate, 20\u2009Hz; 256 \u00d7 256 pixels. e, Vipp1 sheet and spiral filament height offset from the membrane. f\u2013i, Quantification of Vipp1 filament and spiral characteristics. n\u2009=\u2009124, 13, 278, and 278 independent measurements for panels f, g, h, and i, respectively. Error bars show one s.d. of the mean.<\/figcaption><\/figure>\n
*Souvik Naskar, Andrea Merino, Javier Espadas, Jayanti Singh, Aurelien Roux, Adai Colom and Harry H. Low Open Access The article \u201cMechanism for Vipp1 spiral formation, ring biogenesis, and membrane repair<\/em>\u201d by Souvik Naskar, Andrea Merino, Javier Espadas, Jayanti Singh, Aurelien Roux, Adai Colom and Harry H. Low is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n","protected":false},"excerpt":{"rendered":" The ESCRT-III-like protein Vipp1 couples filament polymerization with membrane remodeling. It assembles planar sheets as well as 3D rings and helical polymers, all implicated in mitigating plastid-associated membrane stress. The architecture of Vipp1 planar sheets and helical polymers remains unknown, as do the geometric changes required to transition between polymeric forms. * In the article … Continue reading Mechanism for Vipp 1 spiral formation, ring biogenesis, and membrane repair<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[479,93,48,898,946,983,989,982,987,50,507,376,988,985,136,250,984,92,523,524,52,26,106,51,121,986,392,393,394,521,395,396],"class_list":{"0":"post-2879","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-news","7":"tag-biological-membranes","8":"tag-biology","9":"tag-biology-afm-probes","10":"tag-cell-membranes","11":"tag-cellular-biology","12":"tag-cellular-microbiology","13":"tag-escrt-iii-systems","14":"tag-escrt-iii-like-protein","15":"tag-filament-polymerization","16":"tag-high-speed-afm","17":"tag-high-speed-atomic-force-microscopy-height-spectroscopy","18":"tag-hs-afm-2","19":"tag-membrane-remodeling","20":"tag-membrane-repair","21":"tag-membrane-structure-and-assembly","22":"tag-molecular-biology","23":"tag-ring-biogenesis","24":"tag-structural-biology","25":"tag-ultra-high-frequency-afm-cantilevers","26":"tag-ultra-high-frequency-afm-probes","27":"tag-ultra-short-afm-cantilevers","28":"tag-ultrafast-scanning","29":"tag-usc-f0-3-k0-3","30":"tag-video-rate-afm","31":"tag-video-rate-atomic-force-microscopy","32":"tag-vipp-1-spiral-formation","33":"tag-afm","37":"tag-395","38":"tag-396"},"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2879","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=2879"}],"version-history":[{"count":5,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2879\/revisions"}],"predecessor-version":[{"id":2885,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2879\/revisions\/2885"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=2879"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=2879"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=2879"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nMechanism for Vipp1 spiral formation, ring biogenesis, and membrane repair<\/strong>
\nNature Structural & Molecular Biology (2024)
\nDOI: https:\/\/doi.org\/10.1038\/s41594-024-01401-8<\/a><\/p>\n