{"id":2741,"date":"2024-07-04T20:43:24","date_gmt":"2024-07-04T19:43:24","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=2741"},"modified":"2024-07-04T20:43:24","modified_gmt":"2024-07-04T19:43:24","slug":"stiffness-mediated-mechanosensations-of-airway-smooth-muscle-cells-on-linear-stiffness-gradient-hydrogels","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/stiffness-mediated-mechanosensations-of-airway-smooth-muscle-cells-on-linear-stiffness-gradient-hydrogels\/","title":{"rendered":"Stiffness Mediated-Mechanosensations of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels"},"content":{"rendered":"

Airflow limitation in obstructive airway disease is characterized by narrowing of the airway lumen from excessive contraction of airway smooth muscle (ASM) and remodeling of the airway wall which includes changes in the extracellular matrix (ECM) of the ASM layer.*<\/p>\n

Previous studies on human airway smooth muscle cells ( hASMC ) have independently assessed the influence of extracellular matrix (ECM) proteins on substrates of supra-physiological stiffnesses, such as tissue culture plastic or glass.*<\/p>\n

While the influence of discrete substrate stiffness on hASMC behavior has been examined, manipulation of both substrate stiffness and ECM proteins simultaneously (as expected in disease) has not been extensively modeled in vitro.*<\/p>\n

In the article \u201cStiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels<\/em>\u201d Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi highlight the interplay and complexities between stiffness and ECM protein type on hASMC mechanosensation, relevant to airway remodelling in obstructive airway diseases.*<\/p>\n

The authors first determined a physiological range of ASM layer stiffness using a porcine airway and used these empirical recordings to inform the fabrication of a linear stiffness gradient platform coated with different ECM proteins.*<\/p>\n

Using this linear stiffness gradient platform, Yong Hwee Tan et al. profiled hASMC morphology, contractile function with alpha-smooth muscle actin (\u03b1SMA) and mechanisms of mechanosensation, specifically with nuclear translocalization of Yes-associated protein (YAP) and lamin-A expression.*<\/p>\n

Yong Hwee Tan et al.\u2019s assessment of hASMC mechanosensation utilized an innovative hydrogel platform delivering a linear stiffness gradient to understand stiffness-mediated cell behavior with an ECM substrate for cellular adhesion. *<\/p>\n

The employment of a stiffness gradient that was designed after empirical measurements performed on ex vivo ASM tissue, enabled the presentation of physiologically relevant stiffnesses to study hASMC behavior.*<\/p>\n

Using this platform, the authors of the article found that hASMC mechanosense underlying mechanical cues more than the types of proteins they are anchored to by screening hASMC morphology, contractile phenotype, and mechanomarker expression, with a few exceptions.*<\/p>\n

While the authors acknowledge that the findings from their study were done using cells from only one donor they still think that their study provides a proof of concept for the relevance of hASMC mechanosensation to ECM stiffness, and is another step in the right direction for understanding the pathophysiological impact of airway remodeling in obstructive diseases and exploring potential avenues for improving therapy through greater fidelity of in vitro platforms that include key concepts of mechanosensation. *<\/p>\n

Yong Hwee Tan et al. wanted to use the same method which is used to assess hydrogel stiffness, namely atomic force microscopy (AFM), to measure ASM stiffness.*<\/p>\n

However, nanoscale measurements of ASM strips by AFM proved to be difficult due to an uneven tissue surface after de-epithelialization (Figure S1C, Supporting Information of the cited article), resulting in false force triggering.<\/p>\n

To validate the translation of stiffness values measured from macroscale compression (ASM strips) to nanoscale indentation (AFM on hydrogels), Yong Hwee Tan et al. fabricated additional hydrogels of four different stiffnesses using well-characterized polyacrylamide and compared the stiffness of hydrogels measured by uniaxial compression tester and atomic force microscopy (Figure S2A, Supporting Information of the cited article).<\/p>\n

The nanoscale stiffness of hydrogels was assessed using an atomic force microscope (AFM) with NanoWorld triangular Pyrex-Nitride<\/a> PNP-TR<\/a> AFM probes (the longer AFM cantilever beam \u2013 CB 2 \u2013 with 200 \u00b5m length was used).<\/p>\n

These AFM tips probed hydrogels immersed in 1 \u00d7 PBS with 2 nN, an approach velocity of 2 \u00b5m s\u22121 and a retraction velocity of 10 \u00b5m s\u22121.<\/p>\n

Young’s modulus was determined from linear portions of contact-generated force curves using a custom-written code in Igor Pro.<\/p>\n

All probe indentations were made in triplicate and averaged for a stiffness measurement in kilopascals (kPa).<\/p>\n

An example force curve is shown in Figure S2B, Supporting Information of the cited article. Validation of a linear stiffness gradient was achieved with eight indentations on the hydrogel, 2 mm away from both edges of the hydrogel and at 1 mm intervals along the stiffness gradient axis. Measurements were plotted against displacement from the hydrogel edge (soft to stiff) (Figure 2B of the cited article).<\/p>\n

\"Figure<\/a>
Figure 2 from Yong Hwee Tan et al. 2024 \u201cStiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels\u201d:
Linear stiffness gradient hydrogel fabrication. A) A schematic of a two-step polymerization process. i) 120 \u00b5L of mixed polyacrylamide (PA) solution (% acrylamide + % bis-acrylamide) was added to the primary mold and left to polymerize under ii) a methacrylated coverslip for 20 min. iii) Wedge-shaped 1\u00b0 gel was removed and flipped for placement of a iv) secondary mold before v) addition of a second 120 \u00b5L PA solution and polymerized under a vi) dichlorodimethylsilane-coated coverslip for 20 min. vii) Removal of coverslip and mold completes the fabrication of bi-layered stiffness gradient hydrogel. viii) the dotted arrow indicating the direction of gradient and atomic force microscopy (AFM) measurement. B) Young’s moduli gradient measured by AFM. Twelve hydrogels were selected (one gel per batch) and assessed for stiffness, yielding a gradient of 4.0 kPa mm\u22121, with a range of 1.7 \u00b1 1.2 to 29.6 \u00b1 4.3 kPa (R2 = 0.998, n = 8). Data are presented as mean \u00b1 SD.<\/figcaption><\/figure>\n

 <\/p>\n

\"Figure<\/a>
Figure S2 from Yong Hwee Tan et al. 2024 \u201cStiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels\u201d:
(A) Correlation of Young\u2019s modulus from macroscale stiffness (UCT) assessment with nanoindentation (AFM), was conducted using cylindrical PA hydrogels of different Acrylamide %\/Bis-acrylamide % derived from Tse and Engler [47] 10 %\/0.06 %, 10 %\/0.1 %, 10 %\/0.15 % and 10 %\/0.3 % (Linear regression, P < 0.0001, R2 = 0.9288, n = 4). Data are presented as mean \uf0b1 SEM. (B) An example force curve from atomic force microscopy with an approach velocity of 2 \u03bcm\/s, until a 2 nN trigger force was registered, and retraction of indenter at 10 \u03bcm\/s.<\/figcaption><\/figure>*Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi
\nStiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels<\/strong>
\nAdvanced Healthcare Materials 2024, 2304254
\nDOI: https:\/\/doi.org\/10.1002\/adhm.202304254<\/a><\/p>\n

The article \u201cStiffness Mediated-Mechanosensation of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels<\/em>\u201d by Yong Hwee Tan, Kimberley C. W. Wang, Ian L. Chin, Rowan W. Sanderson, Jiayue Li, Brendan F. Kennedy, Peter B. Noble and Yu Suk Choi is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n","protected":false},"excerpt":{"rendered":"

Airflow limitation in obstructive airway disease is characterized by narrowing of the airway lumen from excessive contraction of airway smooth muscle (ASM) and remodeling of the airway wall which includes changes in the extracellular matrix (ECM) of the ASM layer.* Previous studies on human airway smooth muscle cells ( hASMC ) have independently assessed the … Continue reading Stiffness Mediated-Mechanosensations of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[11,790,62,10,66,65,228,857,858,852,17,48,862,249,865,214,816,860,866,867,856,261,134,47,424,423,861,853,251,482,864,863,859,430,45,854,458,855,580,230,343,588,583],"class_list":{"0":"post-2741","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-news","7":"tag-afm-cantilever","8":"tag-afm-nanoindentation","9":"tag-afm-probes","10":"tag-afm-tips","11":"tag-afm","14":"tag-airway-smooth-muscle-asm","15":"tag-airway-smooth-muscle-cells","16":"tag-alpha-smooth-muscle-actins","17":"tag-atomic-force-microscopy","18":"tag-biology-afm-probes","19":"tag-biomechanical-cues","20":"tag-biomechanics","21":"tag-cell-behavior","22":"tag-cell-morphology","23":"tag-extracellular-matrix","24":"tag-extracellular-matrix-protein","25":"tag-force-curve","26":"tag-force-curves","27":"tag-healthcare-materials","28":"tag-hydrogels","29":"tag-life-science","30":"tag-life-science-afm-probes","31":"tag-materials-research","32":"tag-materials-science","33":"tag-mechanosensation","34":"tag-mechanotransductions","35":"tag-nanoindentation","36":"tag-nanoscale","37":"tag-nanoscale-indentation","38":"tag-nanoscale-measurements","39":"tag-obstructive-airway-diseases","40":"tag-pnp-tr","41":"tag-pyrex-nitride-afm-probes","42":"tag-rigidity","43":"tag-tissue-stiffness","44":"tag-yap-taz","45":"tag-youngs-modulus","46":"tag-230","47":"tag-343","48":"tag-588","49":"tag-583"},"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2741","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=2741"}],"version-history":[{"count":6,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2741\/revisions"}],"predecessor-version":[{"id":2749,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2741\/revisions\/2749"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=2741"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=2741"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=2741"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}