{"id":2148,"date":"2022-02-09T11:52:57","date_gmt":"2022-02-09T10:52:57","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=2148"},"modified":"2023-04-18T12:59:03","modified_gmt":"2023-04-18T11:59:03","slug":"actin-filaments-on-mica-with-aptes","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/actin-filaments-on-mica-with-aptes\/","title":{"rendered":"Actin filaments on Mica with APTES"},"content":{"rendered":"<p>Courtesy of Prof. Noriyuki Kodera, nanoLSI, Kanazawa University, Japan we could upload two new images and a very nice High Speed Atomic Force Microscopy (HS-AFM) video of <em>Actin filaments on Mica with APTES<\/em> to the <a href=\"https:\/\/www.highspeedscanning.com\/high-speed-scanning-afm-images.html\" target=\"_blank\" rel=\"noopener\">image gallery<\/a> and the <a href=\"https:\/\/www.highspeedscanning.com\/high-speed-scanning-video-%D0%B0ctin-filaments\" target=\"_blank\" rel=\"noopener\">video gallery<\/a> on <a href=\"https:\/\/www.highspeedscanning.com\/\" target=\"_blank\" rel=\"noopener\">https:\/\/www.highspeedscanning.com\/<\/a>.<\/p>\n<p>NanoWorld <a href=\"https:\/\/www.nanoworld.com\/Ultra-Short-Cantilevers-USC-F1.2-k0.15\" target=\"_blank\" rel=\"noopener\">USC-F1.2-k0.15<\/a> Ultra-Short AFM cantilevers [C = 0.15 N\/m; fo = 1200 kHz] were used for the high speed imaging.<\/p>\n<p><iframe loading=\"lazy\" title=\"Actin filaments observed by HS-AFM\" width=\"660\" height=\"495\" src=\"https:\/\/www.youtube.com\/embed\/hcggXKDrhBM?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n<figure id=\"attachment_2155\" aria-describedby=\"caption-attachment-2155\" style=\"width: 615px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2022\/02\/09111734\/Actin-filaments-on-Mica-with-APTES-images-by-Prof-Noriyuki-Kodera-using-NanoWorld-USC-F1.2-k0.15-AFM-probes.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2155\" src=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2022\/02\/09111734\/Actin-filaments-on-Mica-with-APTES-images-by-Prof-Noriyuki-Kodera-using-NanoWorld-USC-F1.2-k0.15-AFM-probes.jpeg\" alt=\"HS-AFM Images of actin filaments on Mica with APTES. Buffer:100 mM KCl, 2 mM MgCl2, 1 mM EGTA, 20 mM Imidazole-HCl, pH7.6 (a) 250\u00d7250 nm2 and (b) 400\u00d7400 nm2. Image courtesy of Prof. Kodera, nanoLSI, Kanazawa University, Japan. NanoWorld USC-F1.2-k0.15 AFM probes from the Ultra-Short Cantilever (USC) series were used for the High Speed Atomic Force Microscopy\" width=\"615\" height=\"325\" data-wp-pid=\"2155\" srcset=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2022\/02\/09111734\/Actin-filaments-on-Mica-with-APTES-images-by-Prof-Noriyuki-Kodera-using-NanoWorld-USC-F1.2-k0.15-AFM-probes.jpeg 615w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2022\/02\/09111734\/Actin-filaments-on-Mica-with-APTES-images-by-Prof-Noriyuki-Kodera-using-NanoWorld-USC-F1.2-k0.15-AFM-probes-300x159.jpeg 300w\" sizes=\"auto, (max-width: 615px) 100vw, 615px\" \/><\/a><figcaption id=\"caption-attachment-2155\" class=\"wp-caption-text\">HS-AFM Images of actin filaments on Mica with APTES. Buffer:100 mM KCl, 2 mM MgCl2, 1 mM EGTA, 20 mM Imidazole-HCl, pH7.6 (a) 250\u00d7250 nm2 and (b) 400\u00d7400 nm2. Images courtesy of Prof. Kodera, nanoLSI, Kanazawa University, Japan. NanoWorld USC-F1.2-k0.15 AFM probes were used for the High Speed Atomic Force Microscopy<\/figcaption><\/figure>\n<p>The HighSpeedScanning webpage is dedicated to presenting information about ultra high frequency AFM probe solutions for High Speed AFM ranging from already commercialized AFM probes such as the <a href=\"https:\/\/www.nanoworld.com\/ultra-high-frequency-afm-tips\" target=\"_blank\" rel=\"noopener\">Arrow<sup>TM<\/sup> UHF<\/a> and <a href=\"https:\/\/www.nanoworld.com\/ultra-short-cantilevers-afm-tips\" target=\"_blank\" rel=\"noopener\">NanoWorld Ultra-Short Cantilever (USC) series<\/a> to AFM probes that are still under development.<\/p>\n<p>Additionally to the High-Speed AFM images and videos researchers worldwide kindly have provided us with so that we can share them with the whole High Speed AFM community you will also find\u00a0 a list of <a href=\"https:\/\/www.highspeedscanning.com\/hs-afm-links.html\">links<\/a> and <a href=\"https:\/\/www.highspeedscanning.com\/hs-afm-references.html\" target=\"_blank\" rel=\"noopener\">references<\/a> dedicated to High-Speed AFM on <a href=\"https:\/\/www.highspeedscanning.com\/\">https:\/\/www.highspeedscanning.com\/high-speed-scanning.html\u00a0<\/a><\/p>\n<p>We are aware that these lists are far from complete and we are constantly working on keepting them up to date.\u00a0 If your research institute or company is working with High Speed AFM (HS-AFM) using our AFM probes or if you have published articles with images that were achieved with our High Speed AFM probes annd you find that are missing from our list then please let us know via email: info@highspeedscanning.com if you would like us to add them to the list of references .<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Courtesy of Prof. Noriyuki Kodera, nanoLSI, Kanazawa University, Japan we could upload two new images and a very nice High Speed Atomic Force Microscopy (HS-AFM) video of Actin filaments on Mica with APTES to the image gallery and the video gallery on https:\/\/www.highspeedscanning.com\/. NanoWorld USC-F1.2-k0.15 Ultra-Short AFM cantilevers [C = 0.15 N\/m; fo = 1200 &hellip; <a href=\"https:\/\/www.nanoworld.com\/blog\/actin-filaments-on-mica-with-aptes\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\" >Actin filaments on Mica with APTES<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,2],"tags":[166,96,220,50,25,408,24,431,86,52,26,53,124,51,121,392,393,394,395,396],"class_list":{"0":"post-2148","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-news","7":"category-videos","8":"tag-biochemistry","9":"tag-biophysics","10":"tag-high-frequency-afm-probes","11":"tag-high-speed-afm","12":"tag-high-speed-scanning","13":"tag-high-speed-atomic-force-microscopy","14":"tag-hs-afm","15":"tag-molecular-biochemistry","16":"tag-nanoscale-biophysics","17":"tag-ultra-short-afm-cantilevers","18":"tag-ultrafast-scanning","19":"tag-usc","20":"tag-usc-f1-2-k0-15","21":"tag-video-rate-afm","22":"tag-video-rate-atomic-force-microscopy","23":"tag-afm","26":"tag-395","27":"tag-396"},"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2148","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=2148"}],"version-history":[{"count":10,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2148\/revisions"}],"predecessor-version":[{"id":2161,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/2148\/revisions\/2161"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=2148"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=2148"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=2148"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}