{"id":1493,"date":"2019-09-18T10:59:09","date_gmt":"2019-09-18T09:59:09","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=1493"},"modified":"2023-04-18T12:59:25","modified_gmt":"2023-04-18T11:59:25","slug":"simultaneous-quantification-of-the-interplay-between-molecular-turnover-and-cell-mechanics-by-afm-frap","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/simultaneous-quantification-of-the-interplay-between-molecular-turnover-and-cell-mechanics-by-afm-frap\/","title":{"rendered":"Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP"},"content":{"rendered":"\n

Quantifying the adaptive mechanical behavior of living cells is essential for the understanding of their inner working and function.* <\/p>\n\n\n\n

In their article \u201cSimultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP\u201d Mark Skamrahl, Huw Colin\u2010York, Liliana Barbieri and Marco Fritzsche use a combination of atomic force microscopy and fluorescence recovery after photobleaching is introduced which offers simultaneous quantification and direct correlation of molecule kinetics and mechanics in living cells.* <\/p>\n\n\n\n

Simultaneous quantification of the relationship between molecule kinetics and cell mechanics may thus open up unprecedented insights into adaptive mechanobiological mechanisms of cells.* <\/p>\n\n\n\n

For the AFM nanoindentation tests described in their publication the authors used NanoWorld Arrow-TL2<\/a> tipless cantilevers that were functionalized with a polystyrene bead with 5 \u00b5m radius.*<\/p>\n\n\n\n

\"
Figure 1 a from \u201cSimultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP\u201d by M. Skamrahl et al.:
Establishment and calibration of the optomechanical AFM\u2013FRAP platform. a) Schematic of the AFM\u2013FRAP setup illustrating the experimental power of simultaneous quantification of molecule kinetics and cell mechanics <\/figcaption><\/figure>\n\n\n\n

*Mark Skamrahl, Huw Colin\u2010York, Liliana Barbieri, Marco Fritzsche
Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP<\/strong>
Small 2019, 1902202
DOI: https:\/\/doi.org\/10.1002\/smll.201902202 <\/p>\n\n\n\n

Please follow this external link to the full article https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/smll.201902202 <\/a><\/p>\n\n\n\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\nOpen\nAccess: The article \u00ab Simultaneous Quantification of the Interplay\nBetween Molecular Turnover and Cell Mechanics by AFM\u2013FRAP \u00bb by Mark Skamrahl,\nHuw Colin\u2010York, Liliana Barbieri\nand\nMarco Fritzsche is licensed under a Creative Commons Attribution 4.0\nInternational License, which permits use, sharing, adaptation, distribution and\nreproduction in any medium or format, as long as you give appropriate credit to\nthe original author(s) and the source, provide a link to the Creative Commons\nlicense, and indicate if changes were made. The images or other thirdparty\nmaterial in this article are included in the article\u2019s Creative Commons\nlicense, unless indicated otherwise in a credit line to the material. If\nmaterial is not included in the article\u2019s Creative Commons license and your\nintended use is not permitted by statutory regulation or exceeds the permitted\nuse, you will need to obtain permission directly from the copyright holder. To\nview a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"

Quantifying the adaptive mechanical behavior of living cells is essential for the understanding of their inner working and function.* In their article \u201cSimultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP\u201d Mark Skamrahl, Huw Colin\u2010York, Liliana Barbieri and Marco Fritzsche use a combination of atomic force microscopy and fluorescence recovery after … Continue reading Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM\u2013FRAP<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[252,62,54,162,17,249,97,94,254,248,250,251,16,132,56],"class_list":["post-1493","post","type-post","status-publish","format-standard","hentry","category-news","tag-actin-cytoskeleton","tag-afm-probes","tag-arrow-tipless","tag-arrow-tl2","tag-atomic-force-microscopy","tag-biomechanics","tag-cell-biology","tag-cell-mechanics","tag-kinetics","tag-mechanobiology","tag-molecular-biology","tag-nanoindentation","tag-scanning-probe-microscopy","tag-tipless-cantilever-arrays","tag-tipless-cantilevers"],"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1493","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=1493"}],"version-history":[{"count":8,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1493\/revisions"}],"predecessor-version":[{"id":1502,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1493\/revisions\/1502"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=1493"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=1493"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=1493"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}