{"id":1453,"date":"2019-08-12T02:00:20","date_gmt":"2019-08-12T01:00:20","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=1453"},"modified":"2023-04-18T12:59:25","modified_gmt":"2023-04-18T11:59:25","slug":"effect-of-staple-age-on-dna-origami-nanostructure-assembly-and-stability","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/effect-of-staple-age-on-dna-origami-nanostructure-assembly-and-stability\/","title":{"rendered":"Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability"},"content":{"rendered":"\n<p>DNA origami nanostructures are widely\nemployed in various areas of fundamental and applied research. Due to the\ntremendous success of the DNA origami technique in the academic field,\nconsiderable efforts currently aim at the translation of this technology from a\nlaboratory setting to real-world applications, such as nanoelectronics, drug delivery,\nand biosensing. While many of these real-world applications rely on an intact\nDNA origami shape, they often also subject the DNA origami nanostructures to\nrather harsh and potentially damaging environmental and processing conditions.*<\/p>\n\n\n\n<p>In their\narticle \u201cEffect of Staple Age on DNA Origami Nanostructure Assembly and\nStability\u201d Charlotte Kielar, Yang Xin, Xiaodan Xu, Siqi Zhu, Nelli Gorin ,\nGuido Grundmeier, Christin M\u00f6ser, David M. Smith and Adrian Keller investigate\nthe effect of long-term storage of the employed staple strands on DNA origami\nassembly and stability.*<\/p>\n\n\n\n<p>Atomic\nforce microscopy (AFM) under liquid and dry conditions was employed to\ncharacterize the structural integrity of Rothemund triangles assembled from\ndifferent staple sets that have been stored at \u221220 \u00b0C for up to 43 months.*<\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" aria-label=\"NanoWorld Ultra-Short Cantilevers (opens in a new tab)\" href=\"https:\/\/www.nanoworld.com\/ultra-short-cantilevers-afm-tips\" target=\"_blank\">NanoWorld Ultra-Short Cantilevers<\/a> <a rel=\"noreferrer noopener\" aria-label=\"USC-F0.3-k0.3 (opens in a new tab)\" href=\"https:\/\/www.nanoworld.com\/Ultra-Short-Cantilevers-USC-F0.3-k0.3.html\" target=\"_blank\">USC-F0.3-k0.3<\/a> were the AFM probes that were used for the AFM measurements under liquid conditions.*<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"669\" height=\"1024\" src=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-669x1024.jpg\" alt=\"\" class=\"wp-image-1456\" srcset=\"https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-669x1024.jpg 669w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-196x300.jpg 196w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-768x1176.jpg 768w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-783x1200.jpg 783w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al-409x627.jpg 409w, https:\/\/dhipgo7nn2tea.cloudfront.net\/wp-content\/uploads\/2019\/07\/31165207\/Figure-1-from-Effect-of-Staple-Age-on-DNA-Origami-Nanostructure-Assembly-and-Stability_C_Kielar_et_al.jpg 1200w\" sizes=\"auto, (max-width: 669px) 100vw, 669px\" \/><figcaption>  Figure 1. from \u201c<em>Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability<\/em>\u201d by Charlotte Kielar et al.<br> (a) Schematic illustration of the Rothemund triangle DNA origami. AFM images of DNA origami triangles assembled from staple sets aged for (b) 2\u20137 months, (c) 11\u201316 months, (d) 22\u201327 months, and (e) 38\u201343 months. Measurements were performed either in liquid (left column) or dry conditions after gently dipping the sample into water (central column) or after harsh rinsing (right column). Scale bars represent 250 nm. Height scales are given in the individual images. The insets show zooms of individual DNA origami triangles. <\/figcaption><\/figure>\n\n\n\n<p>*Charlotte\nKielar, Yang Xin, Xiaodan Xu, Siqi Zhu, Nelli Gorin , Guido Grundmeier,\nChristin M\u00f6ser, David M. Smith and Adrian Keller<br>\n<strong>Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability<\/strong><br>\nMolecules 2019, 24(14), 2577<br>\ndoi: https:\/\/doi.org\/10.3390\/molecules24142577<\/p>\n\n\n\n<p>Please follow this external link to the full article: <a href=\"https:\/\/www.mdpi.com\/1420-3049\/24\/14\/2577\/htm\" target=\"_blank\" rel=\"noreferrer noopener\" aria-label=\"https:\/\/www.mdpi.com\/1420-3049\/24\/14\/2577\/htm (opens in a new tab)\">https:\/\/www.mdpi.com\/1420-3049\/24\/14\/2577\/htm<\/a><\/p>\n\n\n\n<p>Open Access: The article \u00ab Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability \u00bb by Charlotte Kielar, Yang Xin, Xiaodan Xu, Siqi Zhu, Nelli Gorin , Guido Grundmeier, Christin M\u00f6ser, David M. Smith and Adrian Keller is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>DNA origami nanostructures are widely employed in various areas of fundamental and applied research. Due to the tremendous success of the DNA origami technique in the academic field, considerable efforts currently aim at the translation of this technology from a laboratory setting to real-world applications, such as nanoelectronics, drug delivery, and biosensing. While many of &hellip; <a href=\"https:\/\/www.nanoworld.com\/blog\/effect-of-staple-age-on-dna-origami-nanostructure-assembly-and-stability\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\" >Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[72,62,10,71,17,93,48,224,105,225,134,47,16,13,226,227,52,53,106,392,393,394,395,396],"class_list":{"0":"post-1453","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-news","7":"tag-afm-in-liquid","8":"tag-afm-probes","9":"tag-afm-tips","10":"tag-afmprobes","11":"tag-atomic-force-microscopy","12":"tag-biology","13":"tag-biology-afm-probes","14":"tag-dna","15":"tag-dna-nanostructures","16":"tag-dna-origami","17":"tag-life-science","18":"tag-life-science-afm-probes","19":"tag-scanning-probe-microscopy","20":"tag-spm-probes","21":"tag-stability","22":"tag-storage","23":"tag-ultra-short-afm-cantilevers","24":"tag-usc","25":"tag-usc-f0-3-k0-3","26":"tag-afm","29":"tag-395","30":"tag-396"},"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=1453"}],"version-history":[{"count":4,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1453\/revisions"}],"predecessor-version":[{"id":1458,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1453\/revisions\/1458"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=1453"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=1453"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=1453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}