{"id":1144,"date":"2019-03-18T11:27:48","date_gmt":"2019-03-18T10:27:48","guid":{"rendered":"https:\/\/www.nanoworld.com\/blog\/?p=1144"},"modified":"2023-04-18T12:59:26","modified_gmt":"2023-04-18T11:59:26","slug":"adhesion-strategies-of-dictyostelium-discoideum-a-force-spectroscopy-study","status":"publish","type":"post","link":"https:\/\/www.nanoworld.com\/blog\/adhesion-strategies-of-dictyostelium-discoideum-a-force-spectroscopy-study\/","title":{"rendered":"Adhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study"},"content":{"rendered":"

“Motile cells require reversible adhesion to solid surfaces to accomplish force transmission upon locomotion. In contrast to mammalian cells, Dictyostelium discoideum ( a soil dwelling amoeba) cells do not express integrins forming focal adhesions but are believed to rely on more generic interaction forces that guarantee a larger flexibility; even the ability to swim has been described for Dictyostelium discoideum (D.d.).”*<\/p>\n

In order to understand the origin of D.d. adhesion, Nadine Kamprad, Hannes Witt, Marcel Schr\u00f6der, Christian Titus Kreis, Oliver B\u00e4umchen, Andreas Janshoff and Marco Tarantola \u00a0describe in their publication \u201cAdhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study<\/strong>\u201d* how they realized and modified a variety of conditions for the amoeba comprising the absence and presence of the specific adhesion protein Substrate Adhesion A (sadA), glycolytic degradation, ionic strength, surface hydrophobicity and strength of van der Waals interactions by generating tailored model substrates. By employing AFM-based single cell force spectroscopy (using NanoWorld Arrow-TL2<\/a> tipless cantilevers) they could show that experimental force curves upon retraction exhibit two regimes described in detail in the article cited above. The study describes a versatile mechanism that allows the cells to adhere to a large variety of natural surfaces under various conditions.<\/span><\/p>\n

\"Fig.<\/a>
Fig. 2 A from “Adhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study” by N. Kamprad et al.: Cell parametrization: \u03b2, angle between the normal on the cell membrane and the cell axis; R1, contact radius between the cell and substrate; R0, equatorial cell radius; R2, contact radius between the cell and cantilever, \u03d51 contact angle towards the substrate; \u03d52, contact angle between the cell and cantilever, in the background is a section of the confocal image in B. B: morphology of the carA-1-GFP labelled D.d. cell attached to the cantilever subjected to a pulling force of 0.2 nN.<\/figcaption><\/figure>\n

*Nadine Kamprad, Hannes Witt, Marcel Schr\u00f6der, Christian Titus Kreis, Oliver B\u00e4umchen, Andreas Janshoff, Marco Tarantola
\nAdhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study
\n<\/strong>Nanoscale, 2018, 10, 22504-22519
\nDOI: 10.1039\/C8NR07107A<\/p>\n

To read the full article follow this external link: https:\/\/pubs.rsc.org\/en\/content\/articlehtml\/2018\/nr\/c8nr07107a<\/a><\/p>\n

Open Access<\/b> The article \u201cAdhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study\u201d by Nadine Kamprad, Hannes Witt, Marcel Schr\u00f6der, Christian Titus Kreis, Oliver B\u00e4umchen, Andreas Janshoff and Marco Tarantola is licensed under a Creative Commons Attribution 3.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/3.0\/<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

“Motile cells require reversible adhesion to solid surfaces to accomplish force transmission upon locomotion. In contrast to mammalian cells, Dictyostelium discoideum ( a soil dwelling amoeba) cells do not express integrins forming focal adhesions but are believed to rely on more generic interaction forces that guarantee a larger flexibility; even the ability to swim has … Continue reading Adhesion strategies of Dictyostelium discoideum \u2013 a force spectroscopy study<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[164,20,54,162,166,165,93,48,96,160,135,167,163,161,56],"class_list":["post-1144","post","type-post","status-publish","format-standard","hentry","category-news","tag-adhesion","tag-arrow-afm-cantilever","tag-arrow-tipless","tag-arrow-tl2","tag-biochemistry","tag-biological-adhesion","tag-biology","tag-biology-afm-probes","tag-biophysics","tag-force-spectroscopy","tag-membrane-biophysics","tag-physical-chemistry","tag-scfs","tag-single-cell-force-spectroscopy","tag-tipless-cantilevers"],"_links":{"self":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1144","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/comments?post=1144"}],"version-history":[{"count":10,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1144\/revisions"}],"predecessor-version":[{"id":1155,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/posts\/1144\/revisions\/1155"}],"wp:attachment":[{"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/media?parent=1144"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/categories?post=1144"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanoworld.com\/blog\/wp-json\/wp\/v2\/tags?post=1144"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}